Coding

Part:BBa_K5335010:Design

Designed by: Lize Chen   Group: iGEM24_HZAU-China   (2024-09-28)


Pxyl-KillerRed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 614
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 259
    Illegal BsaI.rc site found at 521


Design Notes

In our experiments, we coupled the KillerRed protein with the Pxyl promoter (BBa_K143014) and SpovG RBS (BBa_K143021). These elements were cloned into plasmid pHT315 and subsequently transformed into Bacillus subtilis 168 for further examination.


Source

We coupled the KillerRed protein with the Pxyl promoter (BBa_K143014) and SpovG RBS (BBa_K143021).

References